Published: 
Sep 30, 1998

Breast cancer research images

High-resolution copies of images used in this story are linked from the thumbnails below. Please credit the images as indicated.

dewar.tnl.jpg
rwv.tnl.jpg
incubate.tnl.jpg
Dr. Robert Richmond extracts breast cell tissue from one of two liquid nitrogen dewars. Links to . Credit: Dennis Olive, NASA/Marshall Space Flight Center Two of several Bioreactors used by Dr. Richmond in his research. Links to . Credit: Dennis Olive, NASA/Marshall Space Flight Center Breast tissue specimens in traditional sample dishes. Links to . Credit: Dennis Olive, NASA/Marshall Space Flight Center

analyzer.tnl.jpg
spin.tnl.jpg
Dr. Harry Mahtani anlyzes the gas content of nutrient media in the Bioreactors. Links to . Credit: Dennis Olive, NASA/Marshall Space Flight Center Time-lapse exposure of Bioreactor rotation. Links to . Credit: Dennis Olive, NASA/Marshall Space Flight Center

 

 

Human primary breast tumor cells after 56 days of culture in a NASA Bioreactor.

 
becker3.tnl.jpg
 
becker1.tnl.jpg
A cross-section of a construct, grown from surgical specimens of breast cancer, stained for microscopic examination, reveals areas of tumor cells dispersed throughout the non-epithelial cell background. The arrow denotes the foci of breast cancer cells. Links to . Credit: Dr. Jeanne Becker, University of South Florida. Higher magnification of view at left. The arrow points to bead surface indicating breast cancer cells (as noted by the staining of tumor cell intermediate filaments). Links to . Credit: Dr. Jeanne Becker, University of South Florida.

Human primary breast tumor cells after 49 days of growth in a NASA Bioreactor.

becker4.tnl.jpg
becker2.tnl.jpg
Tumor cells aggregate on microcarrier beads (indicated by arrow). Links to . Credit: Dr. Jeanne Becker, University of South Florida. Higher magnification of view at left, illustrating breast cancer cells with intercellular boundaries on bead surface and aggregates of cells achieving 3-dimensional growth outward from bead. Links to . Credit: Dr. Jeanne Becker, University of South Florida.

Isolation of human mammary epithelial cells (HMEC) from breast cancer susceptible tissue
(These are enlarged images from the first composite set on the main story)

4hi-res.tnl.jpg

5hi-res.tnl.jpg

2hi-res.tnl.jpg
B: Outgrowth of cells from duct element in upper right corner cultured in a standard dish; most cells spontaneously die during early cell divisions, but a few will establish long-term growth.
Links to . Credit: Dr. Robert Richmond, NASA/Marshall Space Flight Center.
C: Isolate of long-term growth HMEC from outgrowth of duct element; cells shown soon after isolation and in early full-cell contact growth in culture in a dish.. Links to . Credit: Dr. Robert Richmond, NASA/Marshall Space Flight Center. D: Same long-term growth HMEC, but after 3 weeks in late full-cell contact growth in a continuous culture in a dish. Note attempts to reform duct elements, but this time in two dimensions in a dish rather than in three dimensions in tissue. Links to . Credit: Dr. Robert Richmond, NASA/Marshall Space Flight Center.

Note: Image A appears at full resolution in the linked composite on the first page.